Description
Introduction: Apolipoprotein A2 is a 17.5 kDa protein with a pI of 4.9. Lipids, as such areinsoluble in plasma. They combine with apolipoproteins to form lipoproteinsand this process solubilises lipids in plasma, providing a transport systemfor lipids. Apolipoproteins also play an important role in lipoprotein receptorrecognition and regulation of certain enzymes in lipoprotein metabolism.Two main forms of high density lipoprotein exist; both contain APOA1, butone type only contains APO A2.For more information on APO A2 pleasedownload the PDF catalogue below.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to apo-A2. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation specific for apo-A2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solutionis added to each well. Only those wells that contain apo-A2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit achange in color. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of apo-A2 in the samples is then determined by comparingthe O.D. of the samples to the standard curve